Skip to content Skip to footer
Custom Manufactured Reagents for​
BCR-ABL IS RQ-PCR
  • DESCRIPTION: Single-pot duplex assay with integrated reverse transcription (RT) / cDNA synthesis and qPCR. Minimal Residual Disease determination through BCR-ABL/ABL ratio reported on the International Scale (IS) when used in conjunction with the recommended GenePath Dx RNA extraction protocol
  • SAMPLE TYPE: EDTA peripheral blood or bone marrow
  • REACTION VOLUME: 15 μL
  • LIMIT OF DETECTION: 0.0032% of BCR-ABL fusion transcript with ABL transcript used as the reference gene with a logarithmic reduction of 4.49 in relative expression between BCR-ABL and ABL (MR = 4.49).
  • WORKFLOW: Uses the ‘UNG/dUTP’ system to minimize the risk of PCR carry-over contamination. The ABL transcript serves as a reference and internal control for monitoring the adequacy of sample and RNA extraction
  • INSTRUMENT COMPATIBILITY: Bio-Rad CFX96, Bio-Rad Opus, QIAGEN Rotor-Gene Q, ABI QuantStudio 5, ABI 7500 and Roche Light Cycler 480 II
  • RUN TIME: ~ 100 mins
  • SOFTWARE: Secure online tool to assist with data analysis and calculate the BCR-ABL/ABL ratio as reported on the IS
Custom Manufactured Reagents for​
JAK2 v2 Real Time qPCR
  • DESCRIPTION: Detection of the JAK2 V617F mutation [JAK2:c.G1849T (p.V617F)] in genomic DNA, with standards provided for quantification of % relative Mutant Allele Frequency (MAF)
  • SAMPLE TYPE: EDTA peripheral blood or bone marrow
  • REACTION VOLUME: 15 μL
  • LIMIT OF DETECTION: 1% relative MAF
  • WORKFLOW: Single tube
  • INSTRUMENT COMPATIBILITY: Two channel assay with broad instrument compatibility
  • RUN TIME: ~ 75 mins
  • SOFTWARE: Secure online tool to assist with quantification of % relative MAF and data analysis
Headquarters
674 S. Wagner Road,
Ann Arbor, MI 48103
USA
Tel: +1-734-365-7722
India Office
Manikchand Galleria, 7th Floor, Swastik Society Model Colony, Pune, 411016, Maharashtra, India
Phone: +91 20 7153 1631
Mobile: +91 96073 00010

GenePath Diagnostics © 2023. All Rights Reserved.